Characterization of extracellular vesicles isolated from different liquid biopsies of uveal melanoma patients


  • Carmen Luz Pessuti Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil
  • Deise Fialho Costa Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil and Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec - Canada
  • Kleber S. Ribeiro Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo and Department of Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo - Brazil
  • Mohamed Abdouh Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec - Canada
  • Thupten Tsering Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec - Canada
  • Heloisa Nascimento Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil
  • Alessandra G. Commodaro Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil
  • Allexya Affonso Antunes Marcos Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil
  • Ana Claudia Torrecilhas Department of Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo - Brazil
  • Rubens N. Belfort Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil
  • Rubens Belfort Jr Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil
  • Julia Valdemarin Burnier Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec, Department of Oncology, McGill University, Montreal, Quebec and Experimental Pathology Unit, Department of Pathology, McGill University, Montreal, Quebec - Canada



Aqueous humor, Extracellular vesicles, Liquid biopsy, Plasma, Proteomic analysis, Uveal melanoma, Vitreous humor


Purpose: Uveal melanoma (UM) is the most common intraocular malignant tumor in adults. Extracellular vesicles (EVs) have been extensively studied as a biomarker to monitor disease in patients. The study of new biomarkers in melanoma patients could prevent metastasis by earlier diagnosis. In this study, we determined the proteomic profile of EVs isolated from aqueous humor (AH), vitreous humor (VH), and plasma from UM patients in comparison with cancer-free control patients.

Methods: AH, VH and plasma were collected from seven patients with UM after enucleation; AH and plasma were collected from seven cancer-free patients with cataract (CAT; control group). EVs were isolated using the membrane-based affinity binding column method. Nanoparticle tracking analysis (NTA) was performed to determine the size and concentration of EVs. EV markers, CD63 and TSG101, were assessed by immunoblotting, and the EV proteome was characterized by mass spectrometry.

Results: Mean EV concentration was higher in all analytes of UM patients compared to those in the CAT group. In the UM cohort, the mean concentration of EVs was significantly lower in AH and plasma than in VH. In contrast, the mean size and size distribution of EVs was invariably identical in all analyzed analytes and in both studied groups (UM vs. CAT). Mass spectrometry analyses from the different analytes from UM patients showed the presence of EV markers.

Conclusion: EVs isolated from AH, VH, and plasma from patients with UM showed consistent profiles and support the use of blood to monitor UM patients as a noninvasive liquid biopsy.


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How to Cite

Luz Pessuti, C., Fialho Costa, D. ., Ribeiro, K. S. ., Abdouh, M. ., Tsering, T., Nascimento, H. ., Commodaro, A. G. ., Affonso Antunes Marcos, A. ., Torrecilhas, A. C. ., Belfort, R. N. ., Belfort Jr, R. ., & Valdemarin Burnier, J. (2022). Characterization of extracellular vesicles isolated from different liquid biopsies of uveal melanoma patients. Journal of Circulating Biomarkers, 11(1), 36–47.



Original research article
Received 2022-02-03
Accepted 2022-05-27
Published 2022-06-27


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