Characterization of extracellular vesicles isolated from different liquid biopsies of uveal melanoma patients

Authors

  • Carmen Luz Pessuti Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil https://orcid.org/0000-0002-1776-6235
  • Deise Fialho Costa Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil and Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec - Canada https://orcid.org/0000-0002-1974-4451
  • Kleber S. Ribeiro Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo and Department of Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo - Brazil https://orcid.org/0000-0001-5539-1713
  • Mohamed Abdouh Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec - Canada https://orcid.org/0000-0003-0702-9335
  • Thupten Tsering Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec - Canada https://orcid.org/0000-0002-4613-5790
  • Heloisa Nascimento Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil https://orcid.org/0000-0003-0315-1317
  • Alessandra G. Commodaro Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil https://orcid.org/0000-0002-7816-6508
  • Allexya Affonso Antunes Marcos Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil https://orcid.org/0000-0002-0326-973X
  • Ana Claudia Torrecilhas Department of Pharmaceutical Sciences, Federal University of São Paulo, Diadema, São Paulo - Brazil https://orcid.org/0000-0001-5724-2199
  • Rubens N. Belfort Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil https://orcid.org/0000-0003-1775-7328
  • Rubens Belfort Jr Department of Ophthalmology, Federal University of São Paulo, Vision Institute, IPEPO, São Paulo - Brazil
  • Julia Valdemarin Burnier Cancer Research Program, McGill University Health Centre Research Institute, Montreal, Quebec, Department of Oncology, McGill University, Montreal, Quebec and Experimental Pathology Unit, Department of Pathology, McGill University, Montreal, Quebec - Canada https://orcid.org/0000-0002-2184-2157

DOI:

https://doi.org/10.33393/jcb.2022.2370

Keywords:

Aqueous humor, Extracellular vesicles, Liquid biopsy, Plasma, Proteomic analysis, Uveal melanoma, Vitreous humor

Abstract

Purpose: Uveal melanoma (UM) is the most common intraocular malignant tumor in adults. Extracellular vesicles (EVs) have been extensively studied as a biomarker to monitor disease in patients. The study of new biomarkers in melanoma patients could prevent metastasis by earlier diagnosis. In this study, we determined the proteomic profile of EVs isolated from aqueous humor (AH), vitreous humor (VH), and plasma from UM patients in comparison with cancer-free control patients.

Methods: AH, VH and plasma were collected from seven patients with UM after enucleation; AH and plasma were collected from seven cancer-free patients with cataract (CAT; control group). EVs were isolated using the membrane-based affinity binding column method. Nanoparticle tracking analysis (NTA) was performed to determine the size and concentration of EVs. EV markers, CD63 and TSG101, were assessed by immunoblotting, and the EV proteome was characterized by mass spectrometry.

Results: Mean EV concentration was higher in all analytes of UM patients compared to those in the CAT group. In the UM cohort, the mean concentration of EVs was significantly lower in AH and plasma than in VH. In contrast, the mean size and size distribution of EVs was invariably identical in all analyzed analytes and in both studied groups (UM vs. CAT). Mass spectrometry analyses from the different analytes from UM patients showed the presence of EV markers.

Conclusion: EVs isolated from AH, VH, and plasma from patients with UM showed consistent profiles and support the use of blood to monitor UM patients as a noninvasive liquid biopsy.

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