Circulating erythroblast abnormality associated with systemic pathologies may indicate bone marrow damage


  • Stefan Schreier School of Bioinnovation and Bio-based Product Intelligence, Faculty of Science, Mahidol University, Rama VI Rd, Bangkok, Thailand Center of Excellence in Physics, Ministry of Higher Education, Science, Research and Innovation, Bangkok and Premise Biosystems Co., Ltd. Bangkok - Thailand
  • Prapaphan Budchart Premise Biosystems Co., Ltd. Bangkok - Thailand
  • Suparerk Borwornpinyo Premise Biosystems Co., Ltd. Bangkok and Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, Rama VI, Rd, Bangkok - Thailand
  • Wichit Arpornwirat Department of Oncology, Bangkok Hospital, Bangkok - Thailand
  • Wannapong Triampo Thailand Center of Excellence in Physics, Ministry of Higher Education, Science, Research and Innovation, Bangkok and Department of Physics, Faculty of Science, Mahidol University, Bangkok - Thailand



Bone cancer, Bone marrow damage, Circulating rare cells, Erythroblast, Liquid biopsy


Background: The circulating rare cell population is diverse and rich in diagnostic information. Its characterization and clinical exploitation by cell-based liquid biopsy is an ongoing research task. Bone marrow is one of the major contributors to the peripheral blood rare cell population and, consequently, determines individual rare cell profiles thus depending on bone marrow health status. Bone marrow damage has been associated with aggressive or late-stage systemic diseases and egress of various bone marrow cells into the blood circulation. The association of quantity and heterogeneity of circulating erythroblast with bone marrow damage is of particular interest.

Methods: Circulating CD71high/CD45-/Hoechsthigh blast cells from healthy, noncancer- and cancer-afflicted donors were enriched by CD45 depletion and analyzed by immunofluorescence microscopy.

Results: A new finding of aberrant and mitotic circulating erythroid-like cells that appear similar across blood donors afflicted with various systemic pathologies is reported. Further presented is a classification of said erythroblast-like cells in nine subcategories according to morphological differences between phenotypically similar cells.

Conclusion: Aberrant and mitotic bone marrow-derived rare circulating erythroid-like cells can be detected in the blood of afflicted individuals but not in healthy donors, suggesting the cause of bone marrow damage.


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How to Cite

Schreier, S., Budchart, P., Borwornpinyo, S., Arpornwirat, W., & Triampo, W. (2021). Circulating erythroblast abnormality associated with systemic pathologies may indicate bone marrow damage. Journal of Circulating Biomarkers, 10(1), 14–19.



Short communication
Received 2020-12-07
Accepted 2021-07-12
Published 2021-08-31