Elevated Adiponectin Antibody Levels in Sera of Patients with Atherosclerosis-Related Coronary Artery Disease, Cerebral Infarction and Diabetes Mellitus

Takaki Hiwasa; Xiao-Meng Zhang; Risa Kimura; Mikiko Ohno; Po-Min Chen; Eiichiro Nishi; Koh Ono; Takeshi Kimura; Ikuo Kamitsukasa; Takeshi Wada; Akiyo Aotsuka; Seiichiro Mine; Hirotaka Takizawa; Koichi Kashiwado; Minoru Takemoto; Kazuki Kobayashi; Harukiyo Kawamura; Ryoichi Ishibashi; Koutaro Yokote; Rika Nakamura; Go Tomiyoshi; Natsuko Shinmen; Hideyuki Kuroda

doi:10.33393/jcb.2016.2076

Authors

Takaki Hiwasa Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, Japan
Xiao-Meng Zhang Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, Japan
Risa Kimura Department of Biochemistry and Genetics, Graduate School of Medicine, Chiba University, Chiba, Japan
Mikiko Ohno Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan
Po-Min Chen Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan
Eiichiro Nishi Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan
Koh Ono Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan
Takeshi Kimura Department of Cardiovascular Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan
Ikuo Kamitsukasa Department of Neurology, Chiba Rosai Hospital, Chiba, Japan
Takeshi Wada Department of Internal Medicine, Chiba Aoba Municipal Hospital, Chiba, Japan
Akiyo Aotsuka Department of Internal Medicine, Chiba Aoba Municipal Hospital, Chiba, Japan
Seiichiro Mine Department of Neurological Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan
Hirotaka Takizawa Port Square Kashiwado Clinic, Kashiwado Memorial Foundation, Chiba, Japan
Koichi Kashiwado Department of Neurology, Kashiwado Hospital, Chiba, Japan
Minoru Takemoto Department of Clinical Cell Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan
Kazuki Kobayashi Department of Clinical Cell Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan
Harukiyo Kawamura Department of Clinical Cell Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan
Ryoichi Ishibashi Department of Clinical Cell Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan
Koutaro Yokote Department of Clinical Cell Biology and Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan
Rika Nakamura Medical Project Division, Research Development Center, Fujikura Kasei Co., Saitama, Japan
Go Tomiyoshi Medical Project Division, Research Development Center, Fujikura Kasei Co., Saitama, Japan
Natsuko Shinmen Medical Project Division, Research Development Center, Fujikura Kasei Co., Saitama, Japan
Hideyuki Kuroda Medical Project Division, Research Development Center, Fujikura Kasei Co., Saitama, Japan

DOI:

https://doi.org/10.33393/jcb.2016.2076

Keywords:

Atherosclerosis, Cardiovascular Disease, Cerebral Infarction, Diabetes Mellitus, Antibody Biomarker

Abstract

Adiponectin secreted from the adipocytes plays pleiotropic, anti-atherosclerotic roles, such as enhancement of insulin secretion and an increase in energy expenditure. The measurement of levels of circulating adiponectin is useful to evaluate the progression of atherosclerosis-related diseases, such as coronary artery disease (CAD), cerebral infarction (CI) and diabetes mellitus (DM). We examined the serum antibody levels against recombinant adiponectin protein via the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method. The results revealed that the antibody levels were significantly higher in patients with CAD, CI and type 2 DM, than in healthy donors. Receiver operating curve analysis showed that the sensitivity was in a range of 41–48% for CAD, CI and DM. Thus, the serum anti-adiponectin antibody levels could be a common marker for atherosclerosis-related diseases.