Levofloxacin induces erythrocyte contraction leading to red cell death

Hafiz Muhammad Aslam; Azka Sohail; Ammara Shahid; Maham Abdul Bari Khan; Muhammad Umar Sharif; Razia Kausar; Samia Nawab; Waqas Farooq; Dr. Kashif Jilani; Majeeda Rasheed

doi:10.33393/dti.2024.3060

Authors

Hafiz Muhammad Aslam Department of Biochemistry, University of Agriculture Faisalabad - Pakistan https://orcid.org/0009-0002-5252-1214
Azka Sohail Department of Biochemistry, University of Agriculture Faisalabad - Pakistan
Ammara Shahid Department of Internal Medicine, Amna Anayat Medical College, Sheikhupura - Pakistan
Maham Abdul Bari Khan Department of Biochemistry, University of Agriculture Faisalabad - Pakistan https://orcid.org/0009-0004-6520-5215
Muhammad Umar Sharif Department of Anatomy, Faculty of Veterinary Science, University of Agriculture Faisalabad - Pakistan
Razia Kausar Department of Anatomy, Faculty of Veterinary Science, University of Agriculture Faisalabad - Pakistan
Samia Nawab Department of Chemistry, Government Graduate College For Women, Township - Lahore
Waqas Farooq Institute of Biomedical Sciences, Shanxi University, Taiyuan - China
Kashif Jilani Department of Biochemistry, University of Agriculture Faisalabad - Pakistan
Majeeda Rasheed Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology Rahimyar Khan, Punjab - Pakistan https://orcid.org/0000-0003-1400-9906

DOI:

https://doi.org/10.33393/dti.2024.3060

Keywords:

Eryptosis, Erythrocyte, Hemolysis, Levofloxacin, Oxidative stress

Abstract

Background: Levofloxacin, a fluoroquinolone, is an extensively used antibiotic effective against both positively and negatively staining bacteria. It works by inhibiting bacterial topoisomerase type II and topoisomerase type IV, resulting in impaired DNA synthesis and bacterial cell death. Eryptosis is another term for apoptotic cell death of erythrocyte marked by cell shrinkage, phosphatidylserine (PS) flipping, and membrane blebbing.

Methods: The intent of the present research was to look at the eryptotic effect of levofloxacin by exposing erythrocytes to therapeutical doses (7, 14 µM) of levofloxacin for 48 hours. Cell size evaluation, PS subjection to outside, and calcium channel inhibition were carried out to investigate eryptosis. Oxidative stress generated by levofloxacin was measured as a putative mechanism of eryptosis using glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase activities. Similarly, hemolysis measurements demonstrated levofloxacin’s cytotoxic effect.

Results: Our findings showed that therapeutic doses of levofloxacin can cause a considerable decline in antioxidant enzymes activities, as well as induce cell shrinkage, PS externalization, and hemolysis in erythrocytes. The role of calcium in triggering erythrocyte shrinkage was also confirmed.

Conclusion: In conclusion, our findings showed that the indicated levofloxacin doses caused oxidative stress, which leads to erythrocyte death via eryptosis and hemolysis. These findings emphasize the importance of using levofloxacin with caution and the need for additional research to mitigate these side effects.

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