Current molecular approach for diagnosis of MRSA: a meta-narrative review

Yi Xing Sim; Qiao Wei Lee; Aisha Abushelaibi; Kok-Song Lai; Swee Hua Erin Lim; Sathiya Maran

doi:10.33393/dti.2022.2522

Authors

Yi Xing Sim School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Selangor - Malaysia https://orcid.org/0000-0003-4585-0909
Qiao Wei Lee School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Selangor - Malaysia
Aisha Abushelaibi Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of Technology, Abu Dhabi - United Arab Emirates
Kok-Song Lai Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of Technology, Abu Dhabi - United Arab Emirates
Swee Hua Erin Lim Health Sciences Division, Abu Dhabi Women’s College, Higher Colleges of Technology, Abu Dhabi - United Arab Emirates
Sathiya Maran School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Selangor - Malaysia https://orcid.org/0000-0001-5617-901X

DOI:

https://doi.org/10.33393/dti.2022.2522

Keywords:

Antimicrobial resistance, Molecular diagnosis, MRSA

Abstract

Introduction: Detection and diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) are important in ensuring a correct and effective treatment, further reducing its spread. A wide range of molecular approaches has been used for the diagnosis of antimicrobial resistance (AMR) in MRSA. This review aims to study and appraise widely used molecular diagnostic methods for detecting MRSA.

Methods: This meta-narrative review was performed by searching PubMed using the following search terms: (molecular diagnosis) AND (antimicrobial resistance) AND (methicillin-resistant Staphylococcus aureus). Studies using molecular diagnostic techniques for the detection of MRSA were included, while non-English language, duplicates and non-article studies were excluded. After reviewing the libraries and a further manual search, 20 studies were included in this article. RAMESES publication standard for narrative reviews was used for this synthesis.

Results: A total of 20 full papers were reviewed and appraised in this synthesis, consisting of PCR technique (n = 7), deoxyribonucleic acid (DNA) Microarray (n = 1), DNA sequencing (n = 2), Xpert MRSA/SA BC assay (n = 2), matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) (n = 2), MLST (n = 4), SCCmec typing (n = 1) and GENECUBE (n = 1).

Discussion: Different diagnostic methods used to diagnose MRSA have been studied in this review. This study concludes that PCR has been extensively used due to its higher sensitivity and cost-effectiveness in the past five years

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