Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication

Authors

  • Anne Louise Schacht Revenfeld Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark
  • Evo Kristina Lindersson Søndergaard Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark
  • Allan Stensballe Department of Health Science and Technology, Laboratory for Medical Mass Spectrometry Fredrik Bajersvej, Aalborg University, Aalborg, Denmark
  • Rikke Bæk Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark
  • Malene Møller Jørgensen Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark
  • Kim Varming Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark

DOI:

https://doi.org/10.33393/jcb.2016.2073

Keywords:

Extracellular Vesicles, Cell Communication, Contact-independent, EV Array, Phenotype, Transwell

Abstract

Appropriate and well-documented in vitro cell-culturing systems are necessary to study the activity and biological function of extracellular vesicles (EVs). The aim of this study was to describe an experimental system, in which dynamic, vesicle-based cell communication can be investigated. A commercially available cell-culturing system was applied to study contact-independent cell communication, which separated two cell populations using a membrane with a pore size of 0.4 μm. The EV exchange characteristics between the two compartments in the culture set-up was preliminarily investigated in a cell-free set-up, and analysed using the Extracellular Vesicle (EV) Array and Nanoparticle Tracking Analysis. The application of the cell-culturing set-up was demonstrated using co-cultures of human primary cells. The effects of the relative placement of the two cell populations on the phenotype of EVs found in the cell supernatant were investigated. The results indicate that this placement can be important for the biological hypothesis that is being investigated. These observations are relevant for short (<24h) as well as long (several days) studies of vesicle-based cell communication. Moreover, the introduced cell-culturing set-up and analytical strategy can be used to study contact-independent vesicle communication in a reproducible manner.

Downloads

Download data is not yet available.

Downloads

Published

2016-02-26

How to Cite

Revenfeld, A. L. S., Søndergaard, E. K. L., Stensballe, A., Bæk, R., Jørgensen, M. M., & Varming, K. (2016). Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication. Journal of Circulating Biomarkers, 5(1). https://doi.org/10.33393/jcb.2016.2073

Issue

Section

Original research article

Metrics

Most read articles by the same author(s)