Journal of Circulating Biomarkers https://journals.aboutscience.eu/index.php/jcb Journal of Circulating Biomarkers is an international, peer-reviewed, open access scientific journal focusing on all aspects of the rapidly growing field of circulating blood-based biomarkers and diagnostics using circulating protein and lipid markers, circulating tumor cells (CTC), circulating cell-free DNA (cfDNA) and extracellular vesicles, including exosomes, microvesicles, microparticles, ectosomes and apoptotic bodies. AboutScience srl - Piazza Duca d'Aosta 12 - 20124 Milano MI (Italy) en-US Journal of Circulating Biomarkers 1849-4544 A comparison of inflammatory markers’ potential to predict weight loss in advanced cancer: a prospective observational study https://journals.aboutscience.eu/index.php/jcb/article/view/3510 <p><strong>Background:</strong> Systemic inflammation is crucial in cancer cachexia, but the optimal measurement method remains<br />unclear. This study compares markers of systemic inflammation (MoSI) in predicting weight loss in patients with<br />metastatic cancer.<br /><strong>Methods:</strong> This prospective, observational multi-center study involved patients undergoing radiotherapy for<br />bone metastases. Baseline assessments included demographics, clinical characteristics, previous weight loss,<br />and appetite loss. MoSI included: C-reactive protein (CRP), albumin, white blood cells, neutrophil-to-lymphocyte<br />ratio, monocyte-to-lymphocyte ratio, interleukin-6 (IL-6), modified Glasgow Prognostic Score (mGPS), and<br />Prognostic Nutritional Index. Body weight was recorded at baseline, 3, and 8 weeks post-radiotherapy. Multiple<br />linear regression assessed MoSI’s predictive ability for weight loss, adjusting for previous weight loss, appetite<br />loss, and primary tumour type. Goodness-of-fit was assessed using adjusted R2.<br /><strong>Results:</strong> Out of 574 recruited patients, 540 and 470 were analyzed at 3 and 8 weeks, respectively. The median age<br />(IQR) was 67 (15), 330 (61%) were male, and 397 (74%) had a Karnofsky performance status ≥70. In a base model<br />without MoSI, significant predictors of weight loss at 3 weeks were appetite loss and urological, lung, and gastrointestinal<br />cancer (adjusted R2 of 0.064), while at 8 weeks, urological and lung cancer were significant (adjusted R2<br />of 0.035). At 3 weeks, all MoSI significantly improved the base model, with adjusted R2 between 0.078 and 0.091.<br />At 8 weeks: CRP, mGPS, albumin and IL-6 improved the model; however only CRP and mGPS retained an adjusted<br />R2 of ~0.09.<br /><strong>Conclusions:</strong> All MoSI predicted weight loss, but CRP and mGPS were the most optimal.</p> Ola Magne Vagnildhaug Ragnhild H. Habberstad Øyvind Salvesen Trude R. Balstad Asta Bye Olav Dajani Stein Kaasa Pål Klepstad Tora S. Solheim Copyright (c) 2025 Ola Magne Vagnildhaug, Ragnhild H. Habberstad, Øyvind Salvesen, Trude R. Balstad, Asta Bye, Olav Dajani, Stein Kaasa, Pål Klepstad, Tora S. Solheim https://creativecommons.org/licenses/by-nc/4.0 2025-07-28 2025-07-28 14 1 12 20 10.33393/jcb.2025.3510 Soluble interleukin-33 receptor (sST-2): a novel marker for assessing cardiovascular risk in rheumatoid arthritis https://journals.aboutscience.eu/index.php/jcb/article/view/3175 <p><strong>Background:</strong> Rheumatoid arthritis (RA) is the most common inflammatory rheumatic disease, and it significantly<br />increases the risk of cardiovascular disease and death. The evaluation of cardiovascular risk (CVR) is crucial in<br />these patients, but it may be underestimated using the current criteria, as they do not include nontraditional<br />CVR factors. Soluble ST-2, which is the circulating form of the IL-33 receptor, has been identified as a biomarker<br />for cardiovascular and rheumatic diseases. In this study, we examined the role of sST-2 in assessing CVR in RA.<br /><strong>Methods:</strong> Monocentric, retrospective, observational trial. Inclusion of RA patients on variable DMARD therapy.<br />Analysis of RA disease using established scores (DAS 28, VAS, HFQ), clinical findings (number of swollen and painful<br />joints), and laboratory investigation. Documentation of numerous CVR variables. Quantification of soluble<br />sST-2 by ELISA.<br /><strong>Results:</strong> In total, 129 individuals were included. Soluble sST-2 did neither correlate nor was associated with any<br />variable of RA disease activity. In contrast, significant associations were identified between sST-2 and a number<br />of established CVR markers.<br /><strong>Conclusions:</strong> The data indicates a novel role for sST-2 in CVR prediction in RA.</p> Inga Claus Meike Hoffmeister Constantin Remus werner Dammermann Ourania Gioti Oliver Ritter Daniel Patschan Susann Patschan Copyright (c) 2025 Inga Claus, Meike Hoffmeister, Constantin Remus, werner Dammermann, Ourania Gioti, Oliver Ritter, Daniel Patschan, Susann Patschan https://creativecommons.org/licenses/by-nc/4.0 2025-07-28 2025-07-28 14 1 5 11 10.33393/jcb.2025.3175 Irisin and Insulin Interplay in Thyroid Disorders: A Pilot Study https://journals.aboutscience.eu/index.php/jcb/article/view/3396 <p><strong>Background:</strong> This research was performed to evaluate Irisin and Insulin concentrations in Thyroid patients.<br /><strong>Material and methods:</strong> This investigation was performed as a cross-sectional study within the Biochemistry<br />Department at KMC, Mangalore, and the Central Lab at KMCH-AT, Mangalore. Participants were classified into<br />two cohorts: those having regular thyroid function as well as those having thyroid disorder, including both hypothyroid and hyperthyroid patients, with 28 individuals (n = 28) in each category based on thyroid stimulating<br />hormone (TSH) levels obtained during thyroid dysfunction screenings. Socio-demographic variables like height,<br />weight, and body mass index were calculated, along with the assessment of hypertensive or hypotensive conditions. Insulin levels were quantified using an automated analyzer system. Statistical analyses were performed<br />utilizing Easy-R (EZR) version 1.55, developed by Jichi Medical University in Saitama, Japan. The normal distribution<br />of the parameters was evaluated through normality tests, with t-tests and Kruskal-Wallis tests applied as<br />appropriate.<br /><strong>Results:</strong> Irisin levels significantly declined in hypothyroid individuals while showing an insignificant rise in hyperthyroidism. Insulin levels significantly increased in hyperthyroid patients compared to normal and hypothyroid groups. A positive correlation between insulin and irisin was found in hypothyroidism, while a negative correlation was observed in hyperthyroidism.<br /><strong>Conclusion:</strong> Preliminary findings of this study indicate a potential interdependence between Irisin and thyroid<br />levels. Investigating the interaction between the thyroid profile and irisin can pave the way for considering irisin<br />as a biomarker for novel treatment strategies in thyroid disorders and metabolic conditions.</p> Amisha Malhotra Gayathri Rao Aradhana Marathe Sowmya Ananda Jothi Vinod Chandran Copyright (c) 2025 Vinod Chandran, Amisha Malhotra, Gayathri Rao, Aradhana Marathe https://creativecommons.org/licenses/by-nc/4.0 2025-06-09 2025-06-09 14 1 1 4 10.33393/jcb.2025.3396